The Isolation and Characterization of Novel Mycobacteriophages
نویسندگان
چکیده
1 --------------------------------------------------~ Introduction 3 ~----------------------------------------------= Mycobacteria General Information, _________________ -.::::..3 Mycobacteriophages General Information~ __________________ ~5 Objective ___________________________________ -=8 Materials and Methods ________________________________ --=::..9 Phages _____________________________________ ~ Spot Tests ________________________________ ~9 Isolation 10 '---------------------------~ Screerung~ __________________________ ~l~O Plaque Purification, ____________________________ ~l~l High Titer Stocks _________________ ----:.l=2 Isolation ofDNA _____________________________ ~1.::::..3 Restriction Analysis ofDNA'---___________________ ......;1;;;..;;:;..3 Genomic Analysis, __________________________ ==-15 Mycobacteria'---________________________________ =16 Potential Host Isolation, ______________ ----"'1-=6 Characterization of Potential Host, ___________ .-:1;...;;...7 Genomic DNA Extraction _______________________ -=1-=8 Identification of potential host, _____________________ -----'1;;;....;;..8 Results __________________________________________ --=2;.;::..O The Known Phages __________________ -=20 The Novel Mycobacteriophages ______________ __:2=1 Ho~Bacteria~ ___________________ __:2=1 Conc1usion, _______________________ --=2=2 Works Cited. _______________________ --=2=-..;;.4
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